Shanghai Hengyuan to explain the ELISA kit experimental notes and performance - Database & Sql Blog Articles

EL-C1600N100013-B
WIFI test head can be customized for other specifications
Heng Jiaxing AO3401
Spot Remarks 2017+

Our company offers high-quality ELISA kits that are based on the quantitative sandwich enzyme-linked immunosorbent assay (ELISA) method. We strictly adhere to the principle of "quality first, service first, and reputation first," and have gained widespread recognition from scientific research institutions, universities, enterprises, and foreign-funded companies. Our products are exclusively for research purposes and should not be used directly in clinical, industrial, or other non-research applications. Before purchasing, please consult our sales team for guidance on proper usage; otherwise, we will not be held responsible for any issues that may arise during product use.

ELISA Kit Operating Precautions:

  1. Reagents must be stored as indicated on the label and allowed to reach room temperature before use. Diluted standards should be discarded immediately and not stored.
  2. Unused microtiter plates should be returned to their sealed bag promptly to prevent degradation.
  3. Unused reagents should be properly covered or sealed. Do not mix reagents from different batches, and always check the expiration date before use.
  4. Use disposable tips to avoid cross-contamination. Avoid using pipettes with metal parts when handling stop solution and substrate A/B.
  5. Prepare wash solution in a clean plastic container. Ensure all reagents and samples are thoroughly mixed before use.
  6. After washing, pat the microtiter plate dry completely. Do not place absorbent paper directly into the wells to remove moisture.
  7. Substrate A is volatile, so minimize exposure by keeping the lid closed. Substrate B is light-sensitive and should be protected from light. Avoid contact with skin and handle with care. Read OD values immediately after the experiment is completed.
  8. Ensure consistency in the order of reagent addition to maintain uniform incubation time across all wells.
  9. Follow the instructions precisely for incubation time, volume, and sequence of reagent addition.

ELISA Kit Performance:

  • Sensitivity: The minimum detectable concentration is lower than that of the first standard. The kit shows good linearity with a correlation coefficient (R) of over 0.990 between sample results and expected concentrations.
  • Specificity: The kit does not cross-react with other cytokines, ensuring accurate and reliable results.
  • Repeatability: The coefficient of variation between plates is less than 10%, demonstrating high consistency.

If you need more information about the technology or product details, feel free to contact our sales team. We offer free agent testing and provide professional technical support throughout the process, ensuring you have a smooth and confident purchasing experience. Let us help you achieve accurate and reliable results in your research!

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